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Charlotte the stingray took the world by storm when a North Carolina aquarium announced her immaculate conception in early February.

Now, the Aquarium & Shark Lab in Hendersonville has released an ultrasound video of her pups moving their tails inside her womb and confirmed it will release more information tomorrow.

The team has previously said they don't have a specific due date for the virgin stingray because an immaculate conception has never been observed in the animals before, but said she is pregnant with up to four pups and is expected to give birth soon.




Charlotte the stingray is expected to give birth any day now





The team at the Aquarium & Shark Lab in Hendersonville showed an ultrasound of the pups moving their tails inside Charlotte's womb

The aquarium's uncertainty over when Charlotte is due to give birth stems from the team's inability to know exactly when she conceived, particularly because ultrasounds for stingrays are usually conducted in late-term pregnancies.

The cause of her pregnancy was also a mystery because there weren't any male stingrays in the aquarium with her, but experts said it was most likely attributed to parthenogenesis - the scientific term for virgin birth.

When this happens, the stingray develops a cloned embryo of itself, which can happen when the mammal has been in isolation for a lengthy period of time.




The Aquarium & Shark Lab suggested Charlotte could have been impregnated by a shark, but experts said that's impossible





Stingray experts say Charlotte's 'miracle birth' is most likely due to parthenogenesis - a scientific term for virgin birth

‘They can become pregnant without observed mating with a male in two ways,' Dr. Christopher Lowe, a professor of marine biology and director of the Shark Lab at California State University told Dailymail.com last week.

The first is that ‘many species of sharks and rays can store sperm for at least up to one year,' but Lowe said it is unlikely because Charlotte has been on her own for too long.

‘Turns out that parthenogenesis is more common in sharks and rays than we previously thought, so this is the most likely explanation,' Lowe said.

Depending on the water's temperature, the average stingray's gestation period is about three to four months.




Certain animals are capable of reproducing through 'facultative parthenogenesis' in which the egg is fertilized with cells from the mother rather than by a male 





Researchers say Charlotte's ultrasound shows she's carrying up to four pups

Mercedes Burns, an assistant professor of biological sciences at the University of Maryland, told Dailymail.com that because Charlotte's pregnancy appears to have proceeded normally, UltraXtend kaufen she expects the stingray will give birth in another month or so.

‘The interesting thing about Charlotte is she represents the first time for her species that parthenogenesis has been documented, making it the 15th species where parthenogenesis has been documented in an elasmobranch,' Kevin Feldheim, a researcher at Chicago's Field Museum who is a parthenogenesis expert told Dailymail.com.